Antibiotic Susceptibility Test
We demonstrate a microfluidic agarose channel (MAC) system that reduces the AST assay time for determining MICs by single bacterial time lapse imaging. In single-cell tracking using a microscope, changes in bacterial cells can be detected as soon as cells divide, so antibiotic susceptibility can be determined in 3~4 hours compared to 16 hours when using conventional OD measurement. Rapid AST in microbiology laboratories can have a major impact on the care and outcome of hospitalized patients with infections that require curative antibiotic treatments.
Jungil Choi, Jungheon Yoo, Mincheol Lee, Eun-Geun Kim, Ji Soo Lee, Seungok Lee, Seik Joo, Sang Hoon Song, Eui-Chong Kim, Jung Chan Lee, Hee Chan Kim, Yong-Gyun Jung, Sunghoon Kwon, "A rapid antimicrobial susceptibility test based on single-cell morphological analysis", Science Translational Medicine, Volume 6, Issue 267. [PDF]
"Research highlights: microfluidic analysis of antimicrobial susceptibility", Lab on a Chip, Research Highlight, 2015
"A Faster Way to Diagnose Antibiotic Resistance", Scientific American
, Article, 2014 [PDF]