We developed spatially resolved laser activated cell sorter (SLACS) to conserve spatial information of cells during following analyses, in which cells are isolated in single-cell resolution by laser ablation. Our project aims to develop single-cell genomic, epigenomic, or transcriptomic sequencing methods for analyzing biological information from single-cells isolated through SLACS, adding to their spatial information. Using these methods, we observed spatial heterogeneity of cancer cells and transcriptomic profiles of cancer stem cells in breast cancer. In addition, it has been used to discover biomarkers and to infer genetic evolution by identifying various molecular profiles in different tissues.
