Spatially resolved laser activated cell sorter

We developed spatially resolved laser activated cell sorter (SLACS) to conserve spatial information of cells during following analyses, in which cells are isolated in single-cell resolution by laser ablation. Our project aims to develop single-cell genomic, epigenomic, or transcriptomic sequencing methods for analyzing biological information from single-cells isolated through SLACS, adding to their spatial information. Using these methods, we observed spatial heterogeneity of cancer cells and transcriptomic profiles of cancer stem cells in breast cancer. In addition, it has been used to discover biomarkers and to infer genetic evolution by identifying various molecular profiles in different tissues.

• Sungsik Kim, Amos Chungwon Lee, Han-Byoel Lee, Jinhyun Kim, Yushin Jung, Han Suk Ryu, Yongju Lee,Sangwook Bae, Minju Lee, Kyungmin Lee, Ryong Nam Kim, Woong-Yang Park, Wonshik Han & Sunghoon Kwon (2018). PHLI-seq: constructing and visualizing cancer genomic maps in 3D by phenotype-based high-throughput laser-aided isolation and sequencing. Genome biology
• Amos C. Lee, Yongju Lee, Ahyoun Choi, Han-Byoel Lee, Kyoungseob Shin, Hyunho Lee, Ji Young Kim, Han Suk Ryu, Hoe Suk Kim, Seung Yeon Ryu, Sangeun Lee, Jong-Ho Cheun, Duck Kyun Yoo, Sumin Lee, Hansol Choi, Taehoon Ryu, Huiran Yeom, Namphil Kim, Jinsung Noh, Yonghee Lee, Inyoung Kim, Sangwook Bae, Jinhyun Kim, Wooseok Lee, Okju Kim, Yushin Jung, Changhoe Kim, Seo Woo Song, Yeongjae Choi, Junho Chung, Byung Gee Kim, Wonshik Han & Sunghoon Kwon (2022). Spatial epitranscriptomics reveals A-to-I editome specific to cancer stem cell microniches. Nature communications
• Amos Chungwon Lee (2023). Epitranscriptomics of cancer microniches. Nature review cancer
• Sumin Lee, Gyeongjun Kim, JinYoung Lee, Amos Chungwon Lee & Sunghoon Kwon (2024) Mapping cancer biology in space: applications and perspectives on spatial omics for oncology. Molecular cancer

Based on these accomplishments,the spin-off company Meteor Biotech Inc., has commercialized a state-of-the-art SLACS technology, CosmoSort, which has gained considerable interest among clinicians and scientists worldwide as an innovative solution for spatial omics and clinical sample analysis. The company accelerates the adoption of these biomarker discovery technologies across regions including the Asia, U.S. and Europe.